Abstract Purpose Going further on a broad understanding of nonwork besides family, this study aims to analyze differences between women and men considering work-nonwork conflict (WNWC) in the Brazilian context, investigating time spent in eight nonwork dimensions and the dimensions more affected. Design/methodology/approach The study was quantitative and descriptive. A survey was conducted, based on a validated WNWC scale. The sample consisted of 338 professionals working in Brazil. Data analysis was conducted through descriptive statistics and analysis of variance. Findings Compared to men, women declare higher levels of WNWC considering the eight nonwork dimensions, present greater differences in stress-based conflicts and in more collective dimensions and have marriage or no children associated with more WNWC. Research limitations/implications The study highlights the need to include more nonwork aspects into career and management studies to influence organizational practices and individual choices. The main limitation is the non-probabilistic sample (results not generalizable). Practical implications Know more about WNWC will help organizations to improve lives by creating practices and a cultural environment to preserve women’s and men’s nonwork times. It may also help people to choose places to work for, matching their nonwork needs. Social implications The study reinforces demands from new family arrangements, more couples in dual-career and an aging society: organizations must prepare to have workers that want or need to dedicate time to other interests besides family or children. Originality/value It goes further on a broad understanding of nonwork besides family to understand WNWC and how it may affect differently men and women.

Abstract Proteus mirabilis is one of the main pathogens causing urinary tract infections and sepsis. To our knowledge, this is the first report of a P. mirabilis hosting bla GES. The presence of these genes was determined using PCR and sequencing. We identified the presence of bla GES-1 in all three isolates. In addition, we identified the bla KPC-2 and bla NDM-1 genes in the two strains. These data emphasize the importance of monitoring and surveillance of all enterobacteria. The circulation of P. mirabilis strains carrying bla GES-1 constitutes a new scenario of resistance in this species and should be an epidemiological alert for global health.

Abstract The objective of this study was to characterize the structural and physicochemical properties, the bioactive compounds and the antioxidant activity of freeze-dried açaí pulp (FDAP). The morphology of freeze-dried açaí pulp showed a continuous matrix with a spongey aspect, and the X-ray diffractometry indicated that 96.17% of the molecular structure was in the amorphous state and 3.82% in the crystalline state. Freeze-dried açaí pulp presented an interesting chemical composition of macronutrients, which constituted mainly of lipids (49.14 g/100 g d.b.), proteins (9.19 g/100 g d.b.), total crude fiber (20.29 g/100 g d.b.) and total sugars (2.71 g/100 g d.b.). It also showed great amount of total phenolic compounds (1405.03 mg gallic acid/100 g d.b.) and anthocyanins (127 mg cyanidine-3-glucoside/100 g d.b.), allowing for functional properties allegations. There was significant increase in the water absorption and water solubility indices when the freeze-dried açaí pulp was submitted to a gradual increase in temperature, properties that are adequate for industrial use. Thus, the consumption of freeze-dried açaí pulp should be encouraged and also its use by the food industry in order to develop new food products.

Abstract INTRODUCTION Antibiotic resistance in carbapenemase-producing Klebsiella pneumoniae is acquired and disseminated mainly by plasmids. Therefore, we aimed to investigate the occurrence of carbapenemase genes, analyze the genetic diversity by ERIC-PCR, and examine the most common plasmid incompatibility groups (Incs) in clinical isolates of K. pneumoniae from colonization and infection in patients from a hospital in Brazil. METHODS Twenty-seven isolates of carbapenem-resistant K. pneumoniae were selected and screened for the presence of carbapenemase genes and Incs by PCR, followed by amplicon sequencing. RESULTS The bla KPC and bla NDM genes were detected in 24 (88.8 %) and 16 (59.2 %) of the isolates, respectively. Thirteen isolates (48.1 %) were positive for both genes. The IncFIB (92.6 %) and IncQ (88.8 %) were the most frequent plasmids, followed by IncA/C, IncHI1B, and IncL/M, indicating that plasmid variability existed in these isolates. To our knowledge, this is the first report of IncHI1B in Brazil. We found eight isolates with clonal relationship distributed in different sectors of the hospital. CONCLUSIONS The accumulation of resistance determinants, the variability of plasmid Incs, and the clonal dissemination detected in K. pneumoniae isolates demonstrate their potential for infection, colonization, and the dissemination of different resistance genes and plasmids.

Abstract INTRODUCTION: This study investigated the genetic environment of bla KPC-2 in Klebsiella pnemoniae multi-drug resistant clinical isolates. METHODS: Four carbapenemase gene isolates resistant to carbapenems, collected from infected patients from two hospitals in Brazil, were investigated using polymerase chain reaction and plasmid DNA sequencing. RESULTS: The bla KPC-2 gene was located between ISKpn6 and a resolvase tnpR in the non-Tn4401 element (NTEKPC-IId). It was detected on a plasmid belonging to the IncQ1 group. CONCLUSIONS To our knowledge, this is the first report of the presence of the bla KPC-2 gene in the NTEKPC-IId element carried by plasmid IncQ1 from infections in Brazil.

ABSTRACT: The aim of this study was to investigate the effect of heat stress on 12 bean genotypes through the analysis of their reproductive biology in terms of flowering, pollen viability, meiotic behavior, and production. Plants were grown in a climate chamber at 25-20 °C (day and night) and at a high temperature treatment 37-26 °C (day and night) from the vegetative (V4) development stage to physiological maturity. The experimental design was 2 × 12 factorial arrangement with six replications and the factors consisted of heat treatments and genotypes. In three replications, the number of newly opened flowers was checked daily. At physiological maturity, the following traits were evaluated: percentage of pod set, number of pods, number of viable seeds, number of aborted seeds, 100 seed weight, and seed yield (g per plant). The other three replications were used to collect flowers to create slides to study viability of the pollen grain and analyze the meiotic behavior. The heat treatment factor significantly affected the following traits: total number of pollen grains, number of flowers, number of pods, pod set, number of viable seeds, 100 seed weight, and seed yield. The raised temperature reduced these variables, except for percentage of pod set, and increased meiotic irregularities. The mean values regarding seed yield were 16.39 g per plant for the control treatment and 7.46 g per plant under high temperature. IAC Imperador, FT Nobre, Pérola, BRS Estilo, and IAC Diplomata stood out for higher bean seed yield under increased temperature.

ABSTRACT: Presence of the ALMT1 (aluminum-activated malate transporter) gene confers resistance to aluminum toxicity in Triticum aestivum (common wheat). No resistant cultivars of Triticum turgidum ssp. Durum Desf. (durum wheat) have been registered in Brazil. The aim of this study was to map the ALMT1 through application of the FISH (fluorescence in situ hybridization) technique in five wheat genotypes, common and durum, from the Active Germplasm Bank (AGB) of the Instituto Agronômico (IAC): BH 1146, P19, P33, Anahuac, and IAC 1003. FISH-ALMT1 signals were registered in Anahuac (sensitive) chromosomes and in BH 1146, P19, and P33 (resistant) chromosomes. In the three resistant genotypes, a characteristic double FISH signal was found, located in different chromosomes of the complements: in BH 1146 in chromosome 5D, in P19 in 3B, and in P33 in 6B. This FISH - ALMT1 mapping allows for introgression of aluminum resistance in sensitive cultivars through breeding programs using introgression lines containing these carrier chromosomes.

Abstract Recently, many researches have become available in order to value agroindustrial waste and by-products through processes that exploit these wastes and transform them into new value-added products. Thus, this study aimed to assess the chemical composition (proximate analysis, mineral profile, pectic substances, carbohydrates), fatty acid profile, and bioactive compounds of two major exotic fruit wastes (seeds), marolo and soursop, largely found in one of the richest Brazilian biomes, the Savannah. Soursop and marolo seeds showed significant fat (295.1 and 311.3 g kg-1 d.b., respectively), protein (149.9 and 188.7 g kg-1 d.b., respectively), and fiber (426.7 and 351.1 g kg-1 d.b., respectively) contents. Marolo seeds were found to be the richest in phenolic compounds (32043.1 mg GAE kg-1 d.b.) and also presented the highest DPPH• scavenging activity (91.25%). The major phenolic compound found in marolo and soursop seeds was rutin. Among the fatty acids found in both seeds, the ones with unsaturated chains were predominant, such as oleic acid (48.54% for marolo, and 40.35% for soursop seeds). The seeds analyzed were found to be sources of Cu and Mg. Marolo and soursop seeds were found to be a good source of nutritional compounds and an alternative to obtain compounds of interest to be used in the development of food produce, pharmaceutical, and cosmetic products.

RESUMO Objetivo Investigar a qualidade de vida familiar (QVF) entre famílias brasileiras que têm filhos com deficiência intelectual (DI) moderada. Métodos Estudo transversal, realizado com 50 famílias com filhos com DI moderada de São Carlos, São Paulo, Brasil. Os dados foram coletados por meio de formulários, com informações sociodemográficas, os índices de funcionalidade de Barthel e de Lawton & Brody e a Escala de Qualidade de Vida Familiar do Beach Center. Resultados Os domínios mais fortemente correlacionados com a QVF total foram “interação familiar” (r = 0,870; p < 0,001) e “cuidado dos pais com os filhos” (r = 0,845; p < 0,001). Não houve diferenças na distribuição da QVF em relação às variáveis sociodemográficas investigadas. Observou-se correlação moderada (r = 0,326) e significativa (p = 0,021) entre o índice de funcionalidade de Lawton & Brody e a QVF. O modelo de regressão linear ajustado explicou 10,6% da variabilidade encontrada na QVF (p = 0,021) e mostrou que o aumento de uma unidade no valor do índice de Lawton & Brody representou aumento de 0,092 na QVF. Conclusão A QVF das famílias investigadas encontra-se aquém de outras amostras internacionais. Ações clínicas que fortaleçam o diálogo e a coesão familiar e a construção de um plano terapêutico individualizado podem ser meios efetivos de ajuda a essas famílias.

ABSTRACT Objective To investigate the family quality of life (FQoL) among Brazilian families who have children with moderate intellectual disability (ID). Methods A cross-sectional study was carried out with 50 families who have children with moderate DI from São Carlos, São Paulo, Brazil. Data were collected by questionnaires including sociodemographic information, Barthel and Lawton & Brody functional indexes, and the Beach Center Family Quality of Life Scale. Results The domains “family interaction” (r = 0.870; p < 0.001) and “parenting” (r = 0.845; p < 0.001) were more strongly correlated with the total FQoL. There were no differences in the distribution of FQoL according to the sociodemographic variables investigated. A moderate (r = 0.326) and significant (p = 0.021) correlation was observed between the Lawton and Brody functional index and the FQoL. The adjusted linear regression model explained 10.6% of the variability found in the FQoL (p = 0.021) and showed that the increase of one point in the Lawton and Brody index represented an increase of 0.092 in the FQoL. Conclusion The FQoL of the families investigated is below other international samples. Clinical actions that strengthen dialogue and family cohesion, and the construction of an individualized therapeutic plan can be effective ways to help these families.

ABSTRACT Objective: To assess the strategies used by the Nursing team to minimize medication errors in emergency units. Method: Integrative literature review in the PubMed, BDenf, Cochrane and LILACS databases. Timeless research, without language limitation, performed by peers. Articles published in full that answered the guiding question were included in research. Results: Educational strategies (conducting campaigns, elaborating explanatory manuals, creating a multidisciplinary committee involved in the prevention and reduction of adverse drug events); organizational (meetings, Deviance positive, creation of protocols and changes in the work process) and new technologies (implementation of prescription by computerized system, introduction of the unit doses and of the bar code in the administration of medicines) were evidenced in the studies with the purpose of minimizing medication errors in an emergency unit. Conclusion: The strategies identified were effective in minimizing medication errors in emergency units.

RESUMEN Objetivo: Evaluar las estrategias utilizadas por el equipo de Enfermería para minimizar los errores de medicación en las unidades de emergencia. Método: Revisión integrativa de la literatura realizada en las bases de datos PubMed, BDenf, Cochrane y LILACS. Búsqueda atemporal, sin limitación de idioma, realizada por pares. Se incluyeron en esta investigación artículos publicados en su totalidad que respondieran a la pregunta orientadora. Resultados: Las estrategias educativas (realización de campañas, elaboración de manuales explicativos, creación de una comisión multidisciplinaria involucrada con la prevención y la reducción de los eventos adversos a los medicamentos); organizacionales (reuniones, Deviance positive, creación de protocolos y cambios en el proceso de trabajo) y nuevas tecnologías (la aplicación de prescripciones por un sistema informatizado, la implantación de la dosis unitaria y el código de barras en la administración de medicamentos) fueron evidenciadas en los estudios con la finalidad de minimizar los errores de medicación en la unidad de emergencia. Conclusión: Las estrategias identificadas se mostraron eficaces para minimizar los errores de medicación en las unidades de emergencia.

RESUMO Objetivo: Avaliar as estratégias utilizadas pela equipe de Enfermagem para minimizar os erros de medicação nas unidades de emergência. Método: Revisão integrativa da literatura realizada nas bases de dados PubMed, BDenf, Cochrane e LILACS. Pesquisa atemporal, sem limitação de idioma, realizada por pares. Foram incluídos nesta pesquisa artigos publicados na íntegra que respondessem à pergunta norteadora. Resultados: As estratégias educacionais (realização de campanhas, elaboração de manuais explicativos, criação de comissão multidisciplinar envolvida com a prevenção e redução dos eventos adversos aos medicamentos); organizacionais (reuniões, Deviance positive, criação de protocolos e mudanças no processo de trabalho) e novas tecnologias (implementação de prescrição por sistema informatizado, implantação da dose unitária e do código de barras na administração de medicamentos) foram evidenciadas nos estudos com a finalidade de minimizar os erros de medicação em unidade de emergência. Conclusão: As estratégias identificadas mostraram-se eficazes para minimizar erros de medicação nas unidades de emergência.

RESUMO Objetivo: Avaliar as estratégias utilizadas pela equipe de Enfermagem para minimizar os erros de medicação nas unidades de emergência. Método: Revisão integrativa da literatura realizada nas bases de dados PubMed, BDenf, Cochrane e LILACS. Pesquisa atemporal, sem limitação de idioma, realizada por pares. Foram incluídos nesta pesquisa artigos publicados na íntegra que respondessem à pergunta norteadora. Resultados: As estratégias educacionais (realização de campanhas, elaboração de manuais explicativos, criação de comissão multidisciplinar envolvida com a prevenção e redução dos eventos adversos aos medicamentos); organizacionais (reuniões, Deviance positive, criação de protocolos e mudanças no processo de trabalho) e novas tecnologias (implementação de prescrição por sistema informatizado, implantação da dose unitária e do código de barras na administração de medicamentos) foram evidenciadas nos estudos com a finalidade de minimizar os erros de medicação em unidade de emergência. Conclusão: As estratégias identificadas mostraram-se eficazes para minimizar erros de medicação nas unidades de emergência.

ABSTRACT Objective: To assess the strategies used by the Nursing team to minimize medication errors in emergency units. Method: Integrative literature review in the PubMed, BDenf, Cochrane and LILACS databases. Timeless research, without language limitation, performed by peers. Articles published in full that answered the guiding question were included in research. Results: Educational strategies (conducting campaigns, elaborating explanatory manuals, creating a multidisciplinary committee involved in the prevention and reduction of adverse drug events); organizational (meetings, Deviance positive, creation of protocols and changes in the work process) and new technologies (implementation of prescription by computerized system, introduction of the unit doses and of the bar code in the administration of medicines) were evidenced in the studies with the purpose of minimizing medication errors in an emergency unit. Conclusion: The strategies identified were effective in minimizing medication errors in emergency units.

RESUMEN Objetivo: Evaluar las estrategias utilizadas por el equipo de Enfermería para minimizar los errores de medicación en las unidades de emergencia. Método: Revisión integrativa de la literatura realizada en las bases de datos PubMed, BDenf, Cochrane y LILACS. Búsqueda atemporal, sin limitación de idioma, realizada por pares. Se incluyeron en esta investigación artículos publicados en su totalidad que respondieran a la pregunta orientadora. Resultados: Las estrategias educativas (realización de campañas, elaboración de manuales explicativos, creación de una comisión multidisciplinaria involucrada con la prevención y la reducción de los eventos adversos a los medicamentos); organizacionales (reuniones, Deviance positive, creación de protocolos y cambios en el proceso de trabajo) y nuevas tecnologías (la aplicación de prescripciones por un sistema informatizado, la implantación de la dosis unitaria y el código de barras en la administración de medicamentos) fueron evidenciadas en los estudios con la finalidad de minimizar los errores de medicación en la unidad de emergencia. Conclusión: Las estrategias identificadas se mostraron eficaces para minimizar los errores de medicación en las unidades de emergencia.

Abstract INTRODUCTION: The emergence of New Delhi metallo-β-lactamase (NDM) is concernig because it reduces the antibiotic therapy options for bacterial infections. METHODS: Resistant and virulent genes from an isolate of Klebsiella pneumoniae derived from a patient with sepsis in a hospital in Recife-PE, Brazil, were investigated using PCR and DNA sequencing. RESULTS: bla NDM-1, aac(6’)-Ib-cr and acrB resistance genes, and cps and mrkD virulence genes were detected. CONCLUSIONS To our knowledge, this is the first report on bla NDM-1 in Recife-PE. This detection alerts researchers to the need to control the spread of bla NDM-1 resistance gene by this bacterium in Brazil.

Abstract INTRODUCTION: The objective of this study was to characterize genes of aminoglycoside modifying enzymes (AMEs) in colonizing and infecting isolates of E. aerogenes harboring bla KPC from patients at a public hospital in Recife-PE, Brazil. METHODS: We analyzed 29 E. aerogenes clinical isolates resistant to aminoglycosides. AMEs genes were investigated by PCR and sequencing. RESULTS: Colonizing and infecting isolates mainly presented the genetic profiles aac(3)-IIa/aph(3’)-VI or ant(2”)-IIa/aph(3’)-VI. This is the first report of aph(3’)-VI in E. aerogenes harboring bla KPC in Brazil. CONCLUSIONS: The results highlight the importance in establishing rigorous methods for the surveillance of resistance genes, especially in colonized patients.

Resumo Padrões fenológicos de duas populações de Cnidoscolus quercifolius foram avaliados em área de caatinga no Seridó Ocidental, estado da Paraíba, Brasil, no período de março de 2009 a fevereiro de 2011, com dados coletados mensalmente. As avaliações abordaram uma análise quantitativa dos índices de atividade em períodos vegetativo (brotamento e queda) e reprodutivo (floração e frutificação), analisou-se também o índice de intensidade de Borchert, que avaliou o fluxo foliar. As fenofases foram avaliadas em relação a variações de precipitação, potencial de água na planta e fotossistema II. O brotamento ocorreu no fim da estação seca e transição para a estação chuvosa, com pico dessa fenofase em janeiro e novembro de 2010, sendo negativamente correlacionado com a precipitação (rs = -0,54 p <0,05). A queda apresentou pico em outubro de 2009 e 2010, mostrando uma relação direta com o potencial hídrico e eficiência quântica fotossintética. A floração e frutificação tiveram maior sincronia no período de chuvas, a frutificação foi positivamente correlacionada com valores de precipitação durante o período de estudo (rs = 0,56 p <0,05). Os eventos fenológicos de C. quercifolius revelaram marcada sazonalidade em relação à precipitação, o potencial da água e da eficiência quântica do fotossistema II.

Abstract The phenological patterns of two populations of Cnidoscolus quercifolius were evaluated in the western Seridó region of Paraiba state, Brazil, from March 2009 to February 2011, with data collected monthly. The evaluations have addressed the quantitative method of analysis that represents the activity indices for both populations growing seasons (fall and sprout) and reproductive (flowering and fruiting), and also studied the intensity index Borchert, who evaluated the flow leaves. The stages were evaluated with respect to variations in rainfall, water potential of plant and quantum efficiency of photosystem II. The bud in the two populations occurred in the late dry season and transition into the rainy season, with peak leafing in January and November 2010 and was negatively correlated with rainfall (rs = -0.54 p <0.05). Leaf fall showed the peak phase in October 2009 and 2010, showing a direct relationship with water potential and photosynthetic quantum efficiency. The flowering and fruiting had greater synchrony in the seasonal rainy period, fruit set was positively correlated with precipitation values during the study period (rs = 0.56 p <0.05). The phenological events of C. quercifolius show marked seasonality in relation to precipitation, water potential and the quantum efficiency of photosystem II.

Abstract Pequi, murici and sweet passionfruit are typical fruits from Brazilian Cerrado, which stand out for their sensory attributes such as color, flavor and aroma, in addition to their high nutritional value. Their seeds are by-products from the industrial processing of juices, pulps, jellies, and others, and have great exploitation potential due to their high oil content and the presence of bioactive compounds. The present work aimed to evaluate the chemical composition of pequi almonds, and of murici and sweet passionfruit seeds, and also to quantify the total phenolic compounds, carotenoids, anthocyanins and antioxidant activity by the scavenging activity of •DPPH method. Concerning the chemical composition, pequi almonds presented the highest concentration of lipids (50%), followed by sweet passionfruit (30%) and murici seeds (15%). The almonds from pequi fruit showed the greatest content of protein (33.3%) followed by sweet passionfruit seeds (15%), which presented the highest amount of fiber (41.3%). Murici seeds exhibited the highest content of carbohydrates (46.4%). Pequi almonds had the greatest content of the following minerals, potassium, magnesium, sulfur, manganese, zinc, copper, iron, and sodium, and anthocyanins (14.4 mg CYG/100 g d.b.). Sweet passionfruit seeds presented the greatest amount of calcium, and also had the highest carotenoid content (9 mg/100 g d.b.). Murici seeds showed the highest potential for bioactive compounds, with 404 mg GAE/100 g d.b. and 81% of scavenging activity for the •DPPH. The results demonstrated that the studied seeds have the potential to be sources for new product development, such as oil.